Ecological distribution of Legionella pneumophila

Conference · Tue Jan 01 04:00:00 EST 1980

OSTI ID:5309160

Fliermans, C B; Cherry, W B; Orrison, L H; Smith, S J; Tison, D L; Pope, D H

Bacteria were concentrated 500-fold from 20-liter water samples collected from 67 different lakes and rivers in the United States. The data suggest that Legionella pneumophila is part of the natural aquatic environment, and that the bacterium is capable of surviving extreme ranges of environmental conditions. The data further demonstrate the effectiveness of the direct fluorescent antibody technique for detecting L. pneumophila in natural aquatic systems. Smears of the concentrated samples were screened microscopically for the serogroups of L. pneumophilia by the direct fluorescent antibody (DFA) technique. Virtually all of the 793 samples were DFA-positive. Those samples (318) containing the largest numbers of DFA positive bacteria which were morphologically consistent with L. pneumophila were injected into guinea pigs for attempted isolations. Isolates were obtained from habitats with a wide range of physical, chemical, and biological parameters. Samples collected monthly from a thermally altered lake demonstrated a seasonality of guinea pig infection, with greatest infection occurring during the summer months.

• Research Organization: Du Pont de Nemours (E.I.) and Co., Aiken, SC (USA). Savannah River Lab.; Center for Disease Control, Altanta, GA (USA); Rensselaer Polytechnic Inst., Troy, NY (USA). Dept. of Biology
• DOE Contract Number: AC09-76SR00001
• OSTI ID: 5309160
• Report Number(s): DP-MS-80-21; CONF-800554-1
• Country of Publication: United States
• Language: English

Related Subjects

520100* -- Environment
Aquatic-- Basic Studies-- (-1989)
54 ENVIRONMENTAL SCIENCES
AQUATIC ECOSYSTEMS
BACTERIA
DATA
DATA COMPILATION
DATA FORMS
DISTRIBUTION
ECOSYSTEMS
GRAPHS
INFORMATION
LAKES
MICROORGANISMS
NORTH AMERICA
NUMERICAL DATA
RIVERS
STREAMS
SURFACE WATERS
TABLES
USA