Serum level of 19-hydroxyandrostenedione during pregnancy and at delivery determined by gas chromatography/mass spectrometry

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Steroids; (USA)

DOI:https://doi.org/10.1016/0039-128X(90)90105-K· OSTI ID:6746873

Osawa, Y; Ohnishi, S; Yarborough, C; Ohigashi, S; Kosaki, T; Hashino, M; Yanaihara, T; Nakayama, T ^[1]

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1. Medical Foundation of Buffalo Research Institute, NY (USA)

19-Hydroxyandrostenedione (19-OHA) is secreted from the adrenal glands in men and women and also from the placenta during pregnancy. It has been found to cause hypertension in animal models. We have synthesized (7,7-2H2)-19-OHA with high deuterium content and, together with (7,7-2H2)A and (9,11-2H2)estrone (E1), have developed a quantitative assay of serum level 19-OHA, A, and E1 using the gas chromatography/mass spectrometry-mass fragmentography method to monitor individual subjects throughout pregnancy. The labeled 19-OHA, used as internal standard, showed only 6.73% of unlabeled compound. Recovery of standard 19-OHA, A, and E1 (5,000 pg each) added to male plasma was 97.4 +/- 2.3%, 96.3 +/- 2.1%, and 100.1 +/- 4.1% (mean +/- SD), respectively; the intraassay coefficient of variation was 2.1%, 3.5%, and 3.8%, respectively. Ten pregnant subjects without complications and 10 pregnant subjects near term with hypertension were selected (with informed consent). The 19-OHA and E1 serum concentrations of maternal venous blood from uncomplicated pregnancies increased significantly as gestation progressed (19-OHA: first trimester, 225 +/- 72; second trimester, 656 +/- 325; third trimester, 1,518 +/- 544 pg/ml), reaching the highest level at delivery (19-OHA: 1,735 +/- 684 pg/ml). Whereas a positive correlation was found between the level of 19-OHA and E1, no apparent change of the A level was observed during pregnancy. Levels of the three steroid hormones in pregnancy complicated by hypertension in the second and third trimester were not found to be significantly different from those of normal pregnancy (19-OHA of hypertensive subjects: second trimester, 762 +/- 349; third trimester, 1,473 +/- 491 pg/ml).

• OSTI ID: 6746873
• Journal Information: Steroids; (USA), Vol. 55:4; ISSN 0039-128X
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANDROSTENEDIONE
BIOASSAY
HYPERTENSION
PATHOGENESIS
BLOOD
DEUTERIUM
FETUSES
GAS CHROMATOGRAPHY
MAN
MASS SPECTROSCOPY
PLACENTA
PREGNANCY
ANDROGENS
ANDROSTANES
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARDIOVASCULAR DISEASES
CHROMATOGRAPHY
DISEASES
FETAL MEMBRANES
HORMONES
HYDROGEN ISOTOPES
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PRIMATES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
STEROID HORMONES
STEROIDS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
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