Radioactive antiglobulin testing with 125I anti-C4 and anti-C3
Journal Article
·
· Transfusion; (United States)
The report describes methods for quantitating C4 and C3 coating of red blood cells using 125I anti-C4 and anti-C3. Two methods of preparing antisera were used: one involving absorption and elution from complement coated cells, and the other employing IgG fractions of antisera prepared on DEAE cellulose. The use of labeled antisera permits analysis of specificity by the highly sensitive technique of radioimmunoelectrophoresis. One problem with the technique is that results indicate uptake of labeled antisera, rather than amounts of coating substances. Nonetheless, the ease of performance and the reproducibility of the method, make the procedure very useful in comparative studies of uptake of C4 and C3 by red blood cells.
- Research Organization:
- Nashville Regional Red Cross Blood Center, TN
- OSTI ID:
- 6746691
- Journal Information:
- Transfusion; (United States), Journal Name: Transfusion; (United States) Vol. 18:4; ISSN TRANA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Estimation of anti-D IgG in red blood cell eluates using the specific radioactivity of 125I-labeled IgG: effect of unlabeled, cytophilic IgG
Anti-galactose antibodies do not bind to normal human red cells
Evidence for immunoglobulin Fc receptor-mediated prostaglandin2 and platelet-activating factor formation by cultured rat mesangial cells
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· Transfusion (Philadelphia); (United States)
·
OSTI ID:5366640
Anti-galactose antibodies do not bind to normal human red cells
Conference
·
Fri Feb 28 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5389502
Evidence for immunoglobulin Fc receptor-mediated prostaglandin2 and platelet-activating factor formation by cultured rat mesangial cells
Journal Article
·
Thu Sep 01 00:00:00 EDT 1988
· J. Clin. Invest.; (United States)
·
OSTI ID:6682018
Related Subjects
560171* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARRIERS
COMPLEMENT
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ERYTHROCYTES
GLOBULINS
IMMUNE SERUMS
IMMUNOGLOBULINS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIONUCLIDE KINETICS
TRACER TECHNIQUES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARRIERS
COMPLEMENT
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ERYTHROCYTES
GLOBULINS
IMMUNE SERUMS
IMMUNOGLOBULINS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIOIMMUNOLOGY
RADIOISOTOPES
RADIONUCLIDE KINETICS
TRACER TECHNIQUES