Estimation of anti-D IgG in red blood cell eluates using the specific radioactivity of 125I-labeled IgG: effect of unlabeled, cytophilic IgG

Journal Article · Thu Jan 01 00:00:00 EST 1981 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1981.21481275992.x· OSTI ID:5366640

Masouredis, S P; Mahan, L C; Sudora, E J; Langley, J W; Victoria, E J

The specific radioactivity of conventionally prepared 125I IgG anti-D eluates is significantly less (from 1/5 to 1/20) than that of the 125I IgG fraction used to prepare the eluate. This discrepancy is due to the release of unlabeled, cytophilic IgG from normal red blood cells during eluate preparation and does not represent an underestimation of the eluate anti-D IgG content. Cytophilic IgG content of eluates plays an important role in reducing the nonimmunologic binding of labeled antibody IgG. The results justify the assumption used in numerous studies that the specific radioactivity of 125I IgG fractions can be used to provide a valid estimate of the anti-D IgG content of eluates.

• OSTI ID: 5366640
• Journal Information: Transfusion (Philadelphia); (United States), Vol. 21:4
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
IMMUNOGLOBULINS
RADIOIMMUNOASSAY
IODINE 125
TRACER TECHNIQUES
CHEMICAL PREPARATION
ERYTHROCYTES
LABELLED COMPOUNDS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOASSAY
RADIOISOTOPES
SYNTHESIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques