Morphologic lesions and acute toxicity in rainbow trout (Salmo gairdneri) treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin

Journal Article · Fri Jan 01 04:00:00 EST 1988 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398809531119· OSTI ID:6744426

Spitsbergen, J M; Kleeman, J M; Peterson, R E

To determine effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on growth, mortality, and morphologic lesions in rainbow trout, juvenile Shasta or Wytheville strain fish, obtained from 4 hatcheries, were administered graded single doses of TCDD, 0.1-125 ..mu..g/kg, ip. TCDD doses of 25 and 125 ..mu..g/kg caused 85% lethality 2-4 wk after treatment. At these high doses, death occurred before body weight loss could be detected. A lower dose of 5 ..mu..g/kg caused decreased growth and cumulative mortality of 20% after 11 wk. Stress associated with netting and weighing the fish at weekly intervals significantly shortened the delay period prior to TCDD-induced lethality. Gross and microscopic lesions were evident in rainbow trout treated with 10 /sup +/g TCDD/kg, but not in fish treated with 1 or 0.1 ..mu..g/kg. Morphologic lesions occurred consistently in epithelial and lymphomyeloid tissues of TCDD-treated fish. Lymphomyeloid lesions included thymic involution, splenic lymphoid depletion, and hypocellularity of hematopoietic tissues in the head kidney and trunk kidney. In association with decreased hematopoiesis, peripheral leukopenia and thrombocytopenia occurred in Shasta strain yearling trout treated with 1 ..mu..g/kg or more TCDD. Regarding epithelial lesion, all 4 hatchery strains treated with 10 ..mu..g/kg or more TCDD showed multifocal necrosis of gastric cardiac glandular mucosa, 3 of 4 hatchery strains showed vacuolar inclusions in exocrine pancreatic cells, and 2 of 4 hatchery strains showed fin necrosis.

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• OSTI ID: 6744426
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Journal Issue: 3 Vol. 23:3; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
BLOOD FORMATION
CHLORINATED AROMATIC HYDROCARBONS
DIOXIN
GROWTH
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
MORPHOLOGICAL CHANGES
MORTALITY
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
TOXICITY