Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Chemical characterization of a polar portion in the neutral fraction derived from airborne particulate extracts responsible for the embryotoxicity in the chicken embryo

Journal Article · · J. Toxicol. Environ. Health; (United States)
Airborne particulate matter was collected with a high-volume air sampler between June 1984 and May 1985 on the roof top of the authors institute. The tar material extracted was separated into six fractions by liquid-liquid partition and silica gel column chromatography. These fractions were then tested for their embryotoxicities by a chicken embryo assay. A moderately polar fraction per weight and a fraction containing polycyclic aromatic hydrocarbons (PAHs) had the greatest toxicity for chicken embryos. When the polar fraction was purified by high-pressure liquid chromatography, the purified fraction was 3.7 times more toxic than the original polar fraction. To determine the responsible components for the toxicity, the purified fraction as well as the original fraction was analyzed by capillary gas chromatography and gas chromatography-mass spectrometry. The characterized components were classified into oxygenated PAHs (containing ketones, quinones, and aldehydes), nitrogen-containing PAHS, diphenyl-substituted aliphatic ketones (or diketones), and esters of aliphatic acids.
Research Organization:
Osaka Prefectural Institute of Public Health (Japan)
OSTI ID:
6744408
Journal Information:
J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 24:4; ISSN JTEHD
Country of Publication:
United States
Language:
English