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Presence of chloride reduces malate production in epidermis during stomatal opening

Journal Article · · Plant Physiol.; (United States)
DOI:https://doi.org/10.1104/pp.61.3.361· OSTI ID:6743931

When stomata of isolated epidermis of Vicia faba are allowed to open in the presence of K/sup +/ and iminodiacetate (an impermeant zwitterion), malate is formed in the epidermis; the increases in malate content follow a nearly linear relationship with stomatal aperture. Stomata of leaf sections of V. faba floated on water during opening also exhibit this relationship. When isolated epidermis is offered KCl, this relationship is not observed and less malate is detected at comparable stomatal apertures. The data indicate that Cl/sup -/, if present at concentrations greater than or equal to 10/sup -5/ eq liter/sup -1/, can partially satisfy the anion requirement of guard cells of V. faba during stomatal opening. Discrepancies between earlier reports on the relative roles Cl/sup -/ and malate play as counterions for K/sup +/ in guard cells of V. faba could now be explained as resulting from variations in the availability of Cl/sup -/ to guard cells.

Research Organization:
Michigan State Univ., East Lansing
OSTI ID:
6743931
Journal Information:
Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 61:3; ISSN PLPHA
Country of Publication:
United States
Language:
English