Availability of chloride affects the balance between potassium chloride and potassium malate in guard cells of Vicia faba L
Electron probe microanalysis for K and Cl and enzymic determination of malate were performed on epidermal strips of Vicia faba L. which had been incubated with 0.1 equivalent of K/sup +/ per liter in the absence or presence of Cl/sup -/. In the absence of Cl/sup -/, iminodiacetate, a presumed impermeant zwitterion, served as anion. With no Cl/sup -/ in the medium, 91% of the K/sup +/ imported into the guard cells during stomatal opening was neutralized by malate production; import of Cl/sup -/ (presumably from the rest of the epidermal tissue) contributed 6%. In the presence of Cl/sup -/, 50% of the necessary negative charges were provided by malate synthesis, 45% by Cl/sup -/ import. Stomatal opening was not obviously affected by the chloride concentration in the incubation medium, but malate production declined roughly linearly with the logarithm of absolute value of Cl/sup -/ between 10/sup -5/ and 10/sup -1/ equivalent per liter.
- Research Organization:
- Michigan State Univ., East Lansing
- OSTI ID:
- 6549696
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 62; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHEMICAL ANALYSIS
CHLORIDES
CHLORINE COMPOUNDS
ELECTRON PROBES
EPIDERMIS
EPITHELIUM
HALIDES
HALOGEN COMPOUNDS
HYDROXY ACIDS
IONS
LEGUMINOSAE
MALIC ACID
OPENINGS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PLANT CELLS
PLANTS
POTASSIUM CHLORIDES
POTASSIUM COMPOUNDS
PROBES
SKIN
STOMATA
TISSUES
VICIA