A sensitive, radiometric assay for lysophosphatidylcholine

Journal Article · Wed Feb 14 23:00:00 EST 1990 · Analytical Biochemistry; (USA)

DOI:https://doi.org/10.1016/0003-2697(90)90251-4· OSTI ID:6743907

Dobmeyer, D J; Corr, P B; Creer, M H ^[1]

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1. Washington Univ. School of Medicine, St. Louis, MO (USA)

To facilitate investigation of the metabolism of lysophosphatidylcholine and choline lysoplasmalogen in small quantities of tissue, a method for the quantification of these phospholipid species that is capable of accurate and reproducible analysis in samples which contain less than 1 nmol of total choline lysophospholipid was developed. The procedure employs chloroform and methanol extraction of phospholipids from isolated tissue with subsequent separation of the choline lysophospholipid fraction by high-performance liquid chromatography. The choline lysophospholipids are then acetylated with (3H)acetic anhydride and the (3H)acetyl-lysophosphatidylcholine product is isolated by thin-layer chromatography and quantified by liquid scintillation counting. The choline lysophospholipid content in the sample is determined from a standard curve constructed from samples containing a known amount of synthetic lysophosphatidylcholine with correction for recovery based on the inclusion of ({sup 14}C)lysophosphatidylcholine as an internal standard.

• OSTI ID: 6743907
• Journal Information: Analytical Biochemistry; (USA), Journal Name: Analytical Biochemistry; (USA) Vol. 185:1; ISSN ANBCA; ISSN 0003-2697
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLATION
ACYLATION
ANHYDRIDES
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHROMATOGRAPHY
COUNTING TECHNIQUES
DOGS
ESTERS
HEART
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MUSCLES
MYOCARDIUM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
RADIOASSAY
SCINTILLATION COUNTING
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES