Restoration of T cell development in RAG-2-deficient mice by functional TCR transgenes

Journal Article · Fri Feb 05 04:00:00 EST 1993 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.8430336· OSTI ID:6738988

Shinkai, Yoichi; Alt, F W ^[1]; Koyasu, Shigeo; Reinherz, E L ^[2]; Nakayama, Keiichi; Murphy, K M; Loh, D Y ^[3]

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1. Children's Hospital, Boston, MA (United States)
2. Dana-Farber Cancer Inst., Boston, MA (United States) Harvard Medical School, Boston, MA (United States)
3. Washington Univ. School of Medicine, St. Louis, MO (United States)

Introduction of TCR[alpha] transgene, TCR[beta] transgene, or both into RAG-2[sup [minus]/[minus]] mice differentially rescues T cell development. RAG-2[sup [minus]/[minus]] mice have small numbers of TCR[sup [minus]]CD4[sup [minus]]CD8[sup [minus]] (double negative, DN) thymocytes that express CD3[gamma][delta][epsilon] and [zeta] proteins intracellularly. Introduction of a TCR[beta] transgene, but not a TCR[alpha] transgene, into the RAG-2[sup [minus]/[minus]] background restored normal number of thymocytes. These cells were CD4[sup +]CD8[sup +] (double positive, DP) and expressed small amounts of surface TCR[beta] chain dimers in association with CD3[gamma][delta][epsilon] but not [zeta]. RAG-2[sup [minus]/[minus]] mice that expressed [alpha] and [beta] TCR transgenes developed both DP and single positive thymocytes. Thus, the TCR[beta] subunit, possibly in association with a novel CD3 complex, participates in the DN to the DP transition. 28 refs., 3 figs.

• OSTI ID: 6738988
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 259:5096; ISSN SCIEAS; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGY
CELL DIFFERENTIATION
GENES
GENETICS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
SOMATIC CELLS
THYMOCYTES