Expression and sequences of T cell antigen receptor beta-chain genes in the thymus at an early stage after sublethal irradiation
The sequential appearance of the thymocyte subpopulations and TCR gene messages occurred in the thymus of AKR mice (H-2k, Mlsa) from 7 to 14 days after sublethal irradiation. The thymocytes on day 7 after irradiation were composed of a large number of CD4+CD8+ blast-like cells and a relatively high proportion of CD4-CD8- cells (15 to 25%) but few CD3highCD4+CD8-/CD4-CD8+ cells. Approximately 22% of the CD4-CD8- cells were CD3high and -27% of the CD3highCD4-CD8- cells (-6% of whole CD4-CD8- cells) were F23.1+. The thymocytes on day 7 expressed a large amount of gamma- and delta-chain gene transcripts but reduced levels of alpha- and beta-chain gene transcripts. The V gene repertoire of 18 functional beta-chain cDNA derived from the thymocytes on day 7 was compared with those of 20 functional beta-chain cDNA derived from the thymocytes on day 14 which were composed of a large number of CD3lowCD4+CD8+ small-sized cells and a small number of CD3highCD4+CD8- cells. It is noteworthy that the distribution of V beta genes expressed in the thymocytes on day 7 was much the same as that in the thymocytes on day 14 but significantly different from that in normal BALB/c thymocytes as previously described. Interestingly, neither V beta 8.1 nor V beta 6 genes, which are important for recognition of the product of the Mlsa locus, was detected in these two cDNA libraries. These results suggest that clonal selection of TCR V beta repertoire, irrespective of positive or negative selection, appears to occur at the early stage of T cell differentiation, i.e., on the blast-like CD4+CD8+ thymocytes.
- Research Organization:
- Kyushu Univ., Fukuoka (Japan)
- OSTI ID:
- 6073184
- Journal Information:
- J. Immunol.; (United States), Vol. 142:10
- Country of Publication:
- United States
- Language:
- English
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Sequential appearance of thymocyte subpopulations and T cell antigen receptor gene messages in the mouse thymus after sublethal irradiation
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LYMPHOCYTES
CELL DIFFERENTIATION
THYMOCYTES
BIOLOGICAL RADIATION EFFECTS
THYMUS
GENE REGULATION
AMINO ACID SEQUENCE
ANTIGENS
DNA
GENES
IMMUNOGLOBULINS
MICE
PHENOTYPE
RADIATION CHIMERAS
RECEPTORS
SUBLETHAL IRRADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
GLOBULINS
IRRADIATION
LEUKOCYTES
LYMPHATIC SYSTEM
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
MOSAICISM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals