Labeling of double-stranded DNA by ROX-dideoxycytosine triphosphate using terminal deoxynucleotidyl transferase and separation by capillary electrophoresis

Journal Article · Wed Nov 30 23:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00095a041· OSTI ID:6737015

Figeys, D; Renborg, A; Dovichi, N J ^[1]

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1. Univ. of Alberta, Edmonton, Alberta (Canada)

Terminal transferase is used to add a single fluorescently labeled dideoxynucleotide to double-stranded DNA prepared by restriction endonuclease action on a bacteriophage. The product is separated by capillary electrophoresis with both hydroxypropylmethylcellulose and non-cross-linked polyacrylamide. The reaction products generate single peaks for each fragment with hydroxypropylmethylcellulose. However, the higher resolution separation produced by non-cross-linked polyacrylamide shows that the product contains two components for each restriction digest fragment. This labeling technique should be useful in restriction fragment length polymorphism studies. 9 refs., 2 figs.

• DOE Contract Number: FG02-91ER61123
• OSTI ID: 6737015
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:23; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
550200 -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
BLOOD VESSELS
BODY
CAPILLARIES
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CELLULOSE
DNA
ELECTROPHORESIS
ENZYMES
FLUORESCENCE
LABELLING
LASERS
LUMINESCENCE
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
PARASITES
POLYMERS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
TRANSFERASES
VIRUSES