Separation of fragments up to 570 bases in length by use of 6% T non-cross-linked polyacrylamide for DNA sequencing in capillary electrophoresis

Journal Article · Tue Nov 15 04:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00094a031· OSTI ID:6731420

Best, N; Arriaga, E; Chen, D Y; Dovichi, N J ^[1]

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1. Univ. of ALberta, Edmonton, Alberta (Canada)

Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification. 49 refs., 5 figs.

• DOE Contract Number: FG02-91ER61123
• OSTI ID: 6731420
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:22; ISSN ANCHAM; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BASES
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CROSS-LINKING
DNA SEQUENCING
ELECTROPHORESIS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
ORGANS
POLYAMIDES
POLYMERIZATION
POLYMERS
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS