Cholinesterase assay for monitoring the kinetics of the JD6. 5 organophosphorus acid anhydrase in detoxification of diisopropylfluorophosphate. Final report, January-June 1988

Technical Report · Mon Jun 01 00:00:00 EDT 1992

OSTI ID:6732553

Yeh, H R; Cheng, T C; DeFrank, J J

In the present studies, cholinesterase was used for monitoring the enzymatic activities of the JD6.5 organophosphorus acid anhydrase. The kinetic data indicated that: (1) the first order of kinetic constants (k) and Vmax values of the enzymatic reactions increased as the concentrations of the enzyme increased; (2) while the half-life (tl/2) of diisopropylfluorophosphate (DFP) hydrolysis decreased as the enzyme concentrations increased; (3) the minimum time required for hydrolysis of 9mM of DFP was 3 min at the concentrations of the enzyme present; Km values of DFP were found to be in range of 5mM; and (4) both MnCl2 and NaCl were found to be required for the optimal activity of the enzyme.

• Research Organization: Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, MD (United States)
• OSTI ID: 6732553
• Report Number(s): AD-A-257540/5/XAB; CRDEC-TR-335
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
BIOCHEMICAL REACTION KINETICS
CHEMICAL WARFARE AGENTS
DETOXIFICATION
BENCH-SCALE EXPERIMENTS
CHOLINESTERASE
COMPILED DATA
HALF-LIFE
HYDROLYSIS
MANGANESE CHLORIDES
MONITORING
OPTIMIZATION
SODIUM CHLORIDES
ALKALI METAL COMPOUNDS
CARBOXYLESTERASES
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ENZYMES
ESTERASES
HALIDES
HALOGEN COMPOUNDS
HYDROLASES
INFORMATION
ISOTOPES
KINETICS
LYSIS
MANGANESE COMPOUNDS
MANGANESE HALIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SODIUM COMPOUNDS
SOLVOLYSIS
TRANSITION ELEMENT COMPOUNDS
WEAPONS
YEARS LIVING RADIOISOTOPES
560300* - Chemicals Metabolism & Toxicology
450100 - Military Technology
Weaponry
& National Defense- Chemical Explosions & Explosives
550200 - Biochemistry