Cholinesterase assay for monitoring the kinetics of the JD6. 5 organophosphorus acid anhydrase in detoxification of diisopropylfluorophosphate. Final report, January-June 1988
In the present studies, cholinesterase was used for monitoring the enzymatic activities of the JD6.5 organophosphorus acid anhydrase. The kinetic data indicated that: (1) the first order of kinetic constants (k) and Vmax values of the enzymatic reactions increased as the concentrations of the enzyme increased; (2) while the half-life (tl/2) of diisopropylfluorophosphate (DFP) hydrolysis decreased as the enzyme concentrations increased; (3) the minimum time required for hydrolysis of 9mM of DFP was 3 min at the concentrations of the enzyme present; Km values of DFP were found to be in range of 5mM; and (4) both MnCl2 and NaCl were found to be required for the optimal activity of the enzyme.
- Research Organization:
- Chemical Research, Development and Engineering Center, Aberdeen Proving Ground, MD (United States)
- OSTI ID:
- 6732553
- Report Number(s):
- AD-A-257540/5/XAB; CRDEC-TR--335
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
450100 -- Military Technology
Weaponry
& National Defense-- Chemical Explosions & Explosives
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
BENCH-SCALE EXPERIMENTS
BIOCHEMICAL REACTION KINETICS
CARBOXYLESTERASES
CHEMICAL REACTIONS
CHEMICAL WARFARE AGENTS
CHLORIDES
CHLORINE COMPOUNDS
CHOLINESTERASE
COMPILED DATA
DATA
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DETOXIFICATION
ENZYMES
ESTERASES
HALF-LIFE
HALIDES
HALOGEN COMPOUNDS
HYDROLASES
HYDROLYSIS
INFORMATION
ISOTOPES
KINETICS
LYSIS
MANGANESE CHLORIDES
MANGANESE COMPOUNDS
MANGANESE HALIDES
MONITORING
NUMERICAL DATA
OPTIMIZATION
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SODIUM CHLORIDES
SODIUM COMPOUNDS
SOLVOLYSIS
TRANSITION ELEMENT COMPOUNDS
WEAPONS
YEARS LIVING RADIOISOTOPES