Expression of Rhizobium leguminosarum CFN42 genes for lipopolysaccharide in strains derived from different R. leguminosarum soil isolates
- Marquette Univ., Milwaukee, WI (USA)
- Eastern Illinois Univ., Charlston (USA)
Two mutant derivatives of Rhizobium leguminosarum ANU843 defective in lipopolysaccharide (LPS) were isolated. The LPSs of both mutants lacked O antigen and some sugar residues of the LPS core oligosaccharides. Genetic regions previously cloned from another Rhizobium leguminosarum wild-type isolate, strain CFN42, were used to complement these mutants. One mutant was complemented to give LPS that was apparently identical to the LPS of strain ANU843 in antigenicity, electrophoretic mobility, and sugar composition. The other mutant was complemented by a second CFN42lps genetic region. In this case the resulting LPS contained O-antigen sugars characteristic of donor strain CFN42 and reacted weakly with antiserum against CFN42 cells, but did not react detectably with antiserum against ANU843 cells. Therefore, one of the CFN42 lps genetic regions specifies a function that is conserved between the two R. leguminosarum wild-type isolates, whereas the other region, at least in part, specifies a strain-specific LPS structure. Transfer of these two genetic regions into wild-type strains derived from R. leguminosarum ANU843 and 128C53 gave results consistent with this conclusion. The mutants derived from strain ANU843 elicited incompletely developed clover nodules that exhibited low bacterial populations and very low nitrogenase activity. Both mutants elicited normally developed, nitrogen-fixing clover nodules when they carried CFN42 lps DNA that permitted synthesis of O-antigen-containing LPS, regardless of whether the O antigen was the one originally made by strain ANU843.
- DOE Contract Number:
- FG09-87ER13810
- OSTI ID:
- 6728081
- Journal Information:
- Journal of Bacteriology; (USA), Vol. 172:2; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPOPOLYSACCHARIDES
GENES
RHIZOBIUM
MOLECULAR BIOLOGY
ENZYME ACTIVITY
MUTANTS
NITROGEN FIXATION
NITROGENASE
SOILS
SYMBIOSIS
BACTERIA
CARBOHYDRATES
ENZYMES
LIPIDS
MICROORGANISMS
NITRO-GROUP DEHYDROGENASES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
POLYSACCHARIDES
SACCHARIDES
550200* - Biochemistry