Regulation of phenolic catabolism in Rhizobium leguminosarum biovar trifolii
- Yale Univ., New Haven, CT (United States)
- Murdoch Univ. (Australia)
In members of the family Rhizobiaceae, many phenolic compounds are degraded by the protocatechuate branch of the {beta}-ketoadipate pathway, In this paper the authors describe a novel pattern of induction of protocatechuate (pca) genes in Rhizobium leguminosarum biovar trifolii. Isolation of pca mutant strains revealed that 4-hydroxybenzoate, quinate, and 4-coumarate are degraded via the protocatechuate pathway. At least three inducers govern catabolism of 4-hydroxybenzoate to succinyl coenzyme A and acetyl coenzyme A. The enzyme that catalyzes the initial step is induced by its substrate, whereas the catabolite {beta}-carboxy-cis, cis-muconate induces enzymes for the upper protocatechuate pathway, and {beta}-ketoadipate elicits expression of the enzyme for a subsequent step, {beta}-ketoadipate succinyl-coenzyme A transferase. Elucidation of the induction pattern relied in part on complementation of mutant Rhizobium strains by known subclones of Acinetobacter genes expressed off the lac promoter in a broad-host-range vector.
- OSTI ID:
- 5699429
- Journal Information:
- Journal of Bacteriology; (United States), Journal Name: Journal of Bacteriology; (United States) Vol. 173:17; ISSN JOBAA; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
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