Effects of lead on viability and intracellular metal content of C6 rat glioma cells

Journal Article · Fri Jan 01 04:00:00 EST 1988 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398809531112· OSTI ID:6727458

Tiffany-Castiglioni, E; Garcia, D M; Wu, J N; Zmudzki, J; Bratton, G R

Cultured C6 rat glioma cells were exposed to lead (Pb) acetate (0, 1, 10, or 100 ..mu..M) for 3-4 d. Cells were analyzed for changes in viability and intracellular lead, iron, and copper concentrations after Pb treatment was discontinued. The results were compared with previous findings on astroglia and oligodendroglia in culture in order to evaluate C6 cultures as a model for Pb toxicity in glia. Viability was measured by three methods on the day Pb was removed from the cells (designated d 0), and 2 and 9 d after Pb treatment was discontinued (designated d 2 and 9). The methods used were trypan blue dye exclusion, total cell counts, and incorporation of (/sup 3/H)-L-leucine into proteins. With respect to Pb and Fe uptake, C6 cells closely resembled immature astroglia in culture. Unlike C6 cells, however, astroglia showed elevations of intracellular Fe and Cu after treatment. Thus, Pb effects on C6 cells resembled those on cultured oligodendroglia and astroglia in some respects but not in others. C6 cells appear to be an adequate model for selected events in glial toxicosis, such as PB-stimulated protein synthesis in oligodendroglia and Pb uptake in astroglia, but not Pb-induced alterations of intracellular Cu and Fe in astroglia. Their use as a model for glial progenitor cells in Pb toxicity studies remains to be determined.

• Research Organization: Texas A and M Univ., College Station (USA)
• OSTI ID: 6727458
• Journal Information: J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 23:2; ISSN JTEHD
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MODELS
CARBOXYLIC ACIDS
CELL CULTURES
COPPER
DISEASES
ELEMENTS
GLIOMAS
IRON
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEAD COMPOUNDS
MAMMALS
METALS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RATS
RODENTS
SENSITIVITY
TOXICITY
TRACER TECHNIQUES
TRANSITION ELEMENTS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES
VIABILITY