Regulation of adenosine 3': 5'-cyclic monophosphate accumulation in glia by alpha-adrenergic agonists

Journal Article · Mon Feb 12 04:00:00 EST 1979 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(79)90163-2· OSTI ID:5008699

McCarthy, K D; de Vellis, J

The ..cap alpha..-adrenergic modulation of cAMP accumulation of glia was studied. The phenomenon occurs in three different glial culture systems: (a) primary surface cultures of glia, (b) reaggregate cultures containing neurons and glia, and (c) C6 glioma cells. The turn-on and turn-off times of ..cap alpha..-adrenergic modulation appear to be in the same time frame as that of agents which normally increase cAMP accumulation. However, even after prolonged treatment periods, refractoriness does not develop to the modulating capacity of ..cap alpha..-adrenergic agents. Clonidine, reported to act as both an ..cap alpha..-adrenergic agonist and antagonist in the central nervous system, was found to interact with glia as an ..cap alpha..-adrenergic agonist. Alpha-adrenergic modulation was not diminished by the addition of ethylene-glycol-bis-(..beta..-amino-ethyl ether)N,N'-tetra-acetic acid (EGTA) to culture media, suggesting that external calcium is not required for this effect. These results illustrate the complexity of glial pharmacology and need for more defined systems which allow the examination of characterized populations of brain cells.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 5008699
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 24:7; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AGE DEPENDENCE
AMINES
AMP
ANIMAL CELLS
AROMATICS
BIOLOGICAL EFFECTS
CELL CULTURES
CHEMICAL REACTIONS
DATA
DOPAMINE
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EXPERIMENTAL DATA
HORMONES
HYDROXY COMPOUNDS
INFORMATION
NERVE CELLS
NORADRENALINE
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
RECEPTORS
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
TUMOR CELLS