Hydrocortisone selectively inhibits IgE-dependent arachidonic acid release from rat peritoneal mast cells
Purified rat mst cells were used to study the effects of antiinflammatory steroids on the release of (1-14C)-arachidonic acid ((1-14C)AA) and metabolites. Mast cell were incubated overnight with glucocorticoids, (1-14C)AA incorporated into cellular phospholipids and the release of (1-14C)AA, and metabolites determined using a variety of secretagogues. Release of (1-14C)AA and metabolites by concanavalin A, the antigen ovalbumin and anti-immunoglobulin E antibody was markedly reduced by glucocorticoid treatment. Neither the total incorporation of (1-14C)AA nor the distribution into phospholipids was altered by hydrocortisone pretreatment. Glucocorticoid pretreatment did not alter (1-14C)AA release stimulated by somatostatin, compound 48/80, or the calcium ionophore, A23187. These data indicate that antiinflammatory steroids selectively inhibit immunoglobulin dependent release of arachidonic acid from rat mast cells. These findings question the role of lipomodulin and macrocortin as general phospholipase inhibitors and suggest that they may be restricted to immunoglobulin stimuli.
- Research Organization:
- Department of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville
- OSTI ID:
- 6727438
- Journal Information:
- Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 27:2; ISSN PRGLB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CORTICOSTEROIDS
GLOBULINS
GLUCOCORTICOIDS
HORMONES
HYDROCORTISONE
HYDROXY COMPOUNDS
IMMUNOGLOBULINS
INHIBITION
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PERITONEUM
PREGNANES
PROTEINS
RATS
RODENTS
SECRETION
SEROUS MEMBRANES
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES