Rapid extraction of secretin from plasma by XAD-2 resin and its application in the radioimmunoassay of secretin

Journal Article · Sat Jul 01 04:00:00 EDT 1978 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(78)90687-5· OSTI ID:6724775

Tai, H H; Chey, W Y

A simple and rapid method for extraction of plasma secretin has been developed. In this method plasma was allowed to percolate through a XAD-2 resin (a copolymer of styrene and divinylbenzene) column, and secretin was eluted by acid methanol in good yield. The column extract showed little interference in the radioimmunoassay as evidenced by the superimposability of the standard curve prepared in the presence of the column extract to that prepared in the buffer alone. Furthermore, the bound-to-total ratios of radioactive secretin were not affected by the presence of column extracts of secretin-free plasma from different subjects. Using the method described, we were able to detect significant increases in plasma secretin levels in both dogs and humans following intraduodenal infusion of 0.05 N hydrochloric acid in much smaller quantities than those reported in the literature.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6724775
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 87:2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
HORMONES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
POLYMERS
PRIMATES
PURIFICATION
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RESINS
SECRETIN
TRACER TECHNIQUES
VERTEBRATES