Autoradiographic localization of a gluten peptide during organ culture of human duodenal mucosa
An 125I-labeled subfraction of Frazer's fraction III (molecular weight, 8,000) was added to the culture medium during organ culture of duodenal biopsies from two patients with celiac disease in exacerbation. The isotope-labeled gluten peptide was localized by autoradiography after 6, 12, and 24 h of culture. At 6 h, labeling was located mainly in the basal layers of the biopsies. The tissue was well preserved. After 12 h in culture, the labeling had spread to the lamina propria and the crypts. A few grains were located over enterocytes and desquamated cells. Moderate histological signs of toxicity were observed. After 24 h, there was marked toxic deterioration, comparable to that seen after culture with alpha-gliadin. Labeling had spread throughout the entire section. There seemed to be no specificity of the binding, for the entire section was affected. Culture with the identical gluten fraction, in the radionegative state, produced histological deterioration comparable to that seen after exposure to the isotope-labeled peptide. Gluten peptides are presented to the target cells in a unique way during organ culture, different from in vivo conditions. This may influence the results when the organ culture method is used to investigate the pathogenesis of celiac disease.
- OSTI ID:
- 6723836
- Journal Information:
- J. Pediatr. Gastroenterol. Nutr.; (United States), Vol. 2:3
- Country of Publication:
- United States
- Language:
- English
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PEPTIDES
AUTORADIOGRAPHY
SMALL INTESTINE
TISSUE CULTURES
BIOLOGICAL LOCALIZATION
DIGESTIVE SYSTEM
DISEASES
GLUTIN
IODINE 125
LABELLED COMPOUNDS
MUCOUS MEMBRANES
PATHOLOGY
PATIENTS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
550901* - Pathology- Tracer Techniques