Enzymatic synthesis of formic acid from H/sub 2/ and CO/sub 2/ and production of hydrogen from formic acid

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260240104· OSTI ID:6723414

Klibanov, A M; Alberti, B N; Zale, S E

Whole cells of Alcaligenes eutrophus (as well as isolated P. oxalaticus formate dehydrogenase and A. eutrophus hydrogenase coupled via nicotinamide adenine dinucleotide or methyl viologen) have been shown to produce H/sub 2/ from formic acid. Immobilization of the cells in the kappa-carrageenan gel greatly enhances their stability at room temperature. The rate of hydrogen production catalyzed by immobilized A. eutrophus has been studied as a function of the concentrations of the cells and formate and also pH. An inhibition by high concentrations of formate has been found. Immobilized cells were also capable of synthesizing formate from H/sub 2/ and bicarbonate. Yields of formate up to 30% have been obtained. The catalytic efficiency of immobilized A. eutrophus cells was compared with that of palladium adsorbed on activated carbon. 35 refs.

• Research Organization: MIT, Cambridge, Mass, USA
• OSTI ID: 6723414
• Journal Information: Biotechnol. Bioeng.; (United States), Vol. 24:1
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
HYDROGEN PRODUCTION
BIOSYNTHESIS
CARBON DIOXIDE
ENZYMES
FORMATES
FORMIC ACID
IMMOBILIZED CELLS
MICROORGANISMS
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHALCOGENIDES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
SYNTHESIS
080106* - Hydrogen- Production- Biosynthesis & Photochemical Processes