Predisposition of (/sup 125/I)5-iodo-2'-deoxyuridine-labeled normal and neoplastic mouse fibroblasts to lysis by normal macrophages
During an investigation into the apparent natural cytotoxicity of normal peritoneal macrophages toward the murine MCA/76-9, MCA/76-45, and MCA/76-64 sarcoma cells in vitro, it was found that the peritoneal macrophages from nine different inbred strains of mice, as well as spleen and tumor-associated macrophages, preferentially lysed (/sup 125/I)5-iodo-2'-deoxyuridine ((/sup 125/I)dUrd)-labeled target cells. However, unlabeled target cells were actively proliferating under the same conditions. Proliferation of target cells occurred independently of the lytic event, as measured by counting residual tumor cells at intervals or by pulsing cultures with tritiated thymidine (( 3H)dThd), both of which parameters were relatively the same in the presence or absence of macrophages. Moreover, macrophage-enhanced lysis of (/sup 125/I)dUrd-labeled cells was not confined to tumor cells since adherent labeled nontransformed C57BL/6J 3T3 cells, mouse embryo fibroblasts, and suspended concanavalin A-stimulated lymphoblasts were also induced to release /sup 125/I faster in the presence than in the absence of macrophages. Prelabeled L1210 lymphoma cells were not lysed by normal macrophages. Lipopolysaccharide (LPS) treatment of resident peritoneal macrophages did not induce a stronger lytic effect than that induced by untreated macrophages, but it did result in macrophage-mediated inhibition of DNA synthesis by tumor cells. LPS-treated thioglycollate-elicited macrophages induced a higher specific /sup 125/I release from tumor cells than the release from untreated macrophages. Tumor target cells prelabeled with the less radiotoxic (131I)dUrd were also more predisposed to the lytic action of normal macrophages, while (3H)dThd-labeled target cells were relatively unaffected by the presence of macrophages.
- Research Organization:
- Jackson Lab., Bar Harbor, ME
- OSTI ID:
- 6722869
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 71:5; ISSN JNCIA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CELL CULTURES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FIBROBLASTS
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
IODODEOXYURIDINE
IODOURACILS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MACROPHAGES
MAMMALS
MICE
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHAGOCYTES
PHAGOCYTOSIS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
RODENTS
SENSITIVITY
SOMATIC CELLS
SPLEEN
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
URACILS
VERTEBRATES