The specificity of lymphokine-activated killer (LAK) cells in vitro: Fresh normal murine tissues are resistant to LAK-mediated lysis

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Journal of Surgical Research; (USA)

DOI:https://doi.org/10.1016/0022-4804(91)90004-6· OSTI ID:6249521

Lefor, A T; Rosenberg, S A ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

We have previously reported that murine splenocytes incubated in the lymphokine interleukin-2 acquire the ability to mediate the lysis of a variety of fresh tumor cells in short-term chromium-51 release assays. This study was designed to evaluate the effect of lymphokine-activated killer (LAK) cells on fresh normal murine tissues. The susceptibility to lysis by LAK cells of single cell suspensions from a variety of murine tissues including lung, kidney, bone marrow, peripheral blood mononuclear cells, intestinal mucosa, liver, and fetus was studied. While kidney, intestinal mucosa, and peripheral blood mononuclear cells were clearly not lysed, there was a very low level of lysis of lung, bone marrow, liver, and fetus in repeated experiments. Separation of the cell suspensions of lung and bone marrow demonstrated much higher lysis of the adherent cell population, corresponding to an increased number of macrophages in the target cell suspension. Macrophages represent a population of cells particularly sensitive to lysis by LAK cells. All of the normal tissues were highly lysable by LAK cells in antibody-dependent cellular cytotoxicity assays in the presence of the appropriate anti-H-2 antibody. In a series of cold target inhibition studies, fresh normal murine kidney, lung, and bone marrow did not inhibit the lysis of the LAK-sensitive tumor target MCA-102, further demonstrating that fresh normal tissues share little if any of the determinant recognized by LAK cells on tumor targets. However, the MCA-105 and MCA-106 tumors, and the YAC cell line, all of which are sensitive to LAK cell lysis, did inhibit the lysis of MCA-102 tumor. These studies suggest that a common determinant is present on LAK-sensitive tissues that is absent or present in very low amounts on fresh normal tissues.

• OSTI ID: 6249521
• Journal Information: Journal of Surgical Research; (USA), Journal Name: Journal of Surgical Research; (USA) Vol. 50:1; ISSN 0022-4804; ISSN JSGRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
EXPERIMENTAL NEOPLASMS
GROWTH FACTORS
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
LYSIS
MACROPHAGES
MAMMALS
MATERIALS
MICE
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPECIFICITY
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES