The isolation and characterization of beta-glucosidase gene and beta-glucosidase of Trichoderma viride: Progress report
We have demonstrated the induction by sophorose of ..beta..-glucosidase and other cellulases in Trichoderma cultures, isolated large quantities of intact RNA from induced and noninduced cultures, isolated large quantities of high molecular weight DNA free from contaminating polysaccarides and obtained about 3000 recombinant clones containing inserts, presumably of restriction enzyme cleaved Trichoderma DNA. Additionally we have examined the effect of sophorose on the ..beta..-glucosidase of Sporotrichum pulverentum, established an in vitro translation system, and prepared enzymes from the bacterium Oerskovia which will aid in attempts to insert ethanol producing genes into Trichoderma. 28 refs., 2 figs.
- Research Organization:
- North Carolina Univ., Chapel Hill (USA)
- DOE Contract Number:
- AS05-80ER10665
- OSTI ID:
- 6722126
- Report Number(s):
- DOE/ER/10665-1; ON: DE87003899
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Isolation and characterization of B-glucosidase gene and B-glucosidase of Trichoderma viride. Progress report
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Related Subjects
09 BIOMASS FUELS
CELLULASE
ENZYME INDUCTION
GLUCOSIDASE
FRACTIONATION
GENES
ELECTROPHORESIS
RECOMBINANT DNA
TRICHODERMA VIRIDE
DNA
ENZYMES
FUNGI
GENE REGULATION
GLYCOSYL HYDROLASES
HYDROLASES
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
SEPARATION PROCESSES
TRICHODERMA
550200* - Biochemistry
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