Dye-dilution cardiac output without blood withdrawal in the conscious rabbit

Journal Article · Tue Aug 01 04:00:00 EDT 1978 · Am. J. Phys.; (United States)

OSTI ID:6720542

Boerboom, L E; Boelkins, J N

Cardiac output can be measured in the conscious rabbit without blood withdrawal by use of a modified dye-dilution method. Blood flows continuously from the central ear artery through a Silastic catheter that passes through a modified cuvette lumen and back into an ear vein. Blood flow through this shunt was 1.5 ml/min. This modified technique was evaluated by comparison with a simultaneously performed cardiac output measurement using radioactive microspheres and by making consecutive dye-dilution measurements at short time intervals. A correlation coefficient of 0.96 was obtained when comparing dye-dilution and microsphere cardiac outputs. The difference between 11 paired measurements was 7.09 +- 5.24% (mean +- SD). Consecutive outputs measured within 3 min of each other by the flow-through technique had a correlation coefficient of 0.99 and a difference between the first and second determinations of 3.52 +- 2.38%. The method permits dye-dilution cardiac output measurement in the conscious rabbit while avoiding the potential error caused by blood withdrawal from small animals.

• Research Organization: Univ. of North Dakota, Grand Forks
• OSTI ID: 6720542
• Journal Information: Am. J. Phys.; (United States), Journal Name: Am. J. Phys.; (United States) Vol. 235:2; ISSN AJPIA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BLOOD FLOW
BODY
CARDIOVASCULAR SYSTEM
DYES
DYNAMIC FUNCTION STUDIES
HEART
ISOTOPE APPLICATIONS
MAMMALS
MEASURING METHODS
MICROSPHERES
ORGANS
PHYSIOLOGY
RABBITS
TRACER TECHNIQUES
VERTEBRATES