Does tuftsin alter phagocytosis by human polymorphonuclear neutrophils

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Inflammation (N.Y.); (United States)

DOI:https://doi.org/10.1007/BF00910723· OSTI ID:6719028

Cooper, M R; DeChatelet, L R; Shirley, P S; Cooper, M R

The physiological significance of the putative phagocytosis-promoting peptide, tuftsin, was investigated by measurement of chemiluminescence generated during phagocytosis and by assay of the uptake of radiolabeled bacteria. Researchers found no differences in either assay when reasearchers compared serum from splenectomized patients (which purportedly lacks tuftsin) with normal serum. Further, there was no difference when serum from splenectomized patients was employed in the presence of absence of exogenous tuftsin. Similar results were obtained under a variety of conditions, utilizing three different challenge particles with varying particle-cell ratios and serum from 20 different splenectomized patients. These results do not agree with the hypothesis that tuftsin plays a major role in promoting phagocytosis.

• Research Organization: Department of Biochemistry, Bowman Gray School of Medicine, Winston-Salem, North Carolina
• OSTI ID: 6719028
• Journal Information: Inflammation (N.Y.); (United States), Journal Name: Inflammation (N.Y.); (United States) Vol. 6:1; ISSN INFLD
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD SERUM
BODY FLUIDS
COMPARATIVE EVALUATIONS
GLOBULINS
IMMUNOGLOBULINS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MATERIALS
MEASURING METHODS
MICROORGANISMS
NEUTROPHILS
ORGANIC COMPOUNDS
PATIENTS
PHAGOCYTOSIS
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
UPTAKE