Bone scintigraphy in evaluating the viability of composite bone grafts revascularized by microvascular anastomoses, conventional autogenous bone grafts, and free non-revascularized periosteal grafts

Journal Article · Thu Jul 01 04:00:00 EDT 1982 · J. Bone Jt. Surg., Am. Vol.; (United States)

OSTI ID:6718410

Berggren, A; Weiland, A J; Ostrup, L T

Researchers studied the value of bone scintigraphy in the assessment of anastomotic patency and bone-cell viability in free bone grafts revascularized by microvascular anastomoses in twenty-seven dogs. The dogs were divided into three different groups, and scintigraphy was carried out using technetium-labeled methylene diphosphonate in composite bone grafts revascularized by microvascular anastomoses, conventional autogenous bone grafts, and periosteal grafts placed in different recipient beds. The viability of the grafts were evaluated by histological examination and fluorescence microscopy after triple labeling with oxytetracycline on the first postoperative day, alizarin complexone on the fourth postoperative day, and DCAF on the eleventh postoperative day. A positive scintiscan within the first week following surgery indicated patent microvascular anastomoses, and histological study and fluorescence microscopy confirmed that bone throughout the graft was viable. A positive scintiscan one week after surgery or later does not necessarily indicate microvascular patency or bone-cell survival, because new bone formed by creeping substitution on the surface of a dead bone graft can result in this finding.

• Research Organization: Johns Hopkins University School of Medicine, Department of Orthopaedic Surgery, Baltimore, MD
• OSTI ID: 6718410
• Journal Information: J. Bone Jt. Surg., Am. Vol.; (United States), Journal Name: J. Bone Jt. Surg., Am. Vol.; (United States) Vol. 64:6; ISSN JBJSA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
COMPARATIVE EVALUATIONS
COUNTING TECHNIQUES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DOGS
GRAFT-HOST REACTION
GRAFTS
HISTOLOGY
HOURS LIVING RADIOISOTOPES
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
ION MICROSCOPY
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEDICINE
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOIMMUNOLOGY
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
SKELETON
SURGERY
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRANSPLANTS
USES
VERTEBRATES
VIABILITY
YEARS LIVING RADIOISOTOPES