Sensitive radioimmunoassays for 0/sup 6/-n-butyldeoxyguanosine, 0/sup 2/-n-butylthymidine and 0/sup 4/-n-butylthymidine

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.5.547· OSTI ID:6717889

Saffhill, R; Strickland, P T; Boyle, J M ^[1]

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1. Christie Hospital and Holt Radium Inst., Manchester (UK). Paterson Labs.

Radioimmunoassays have been developed using monoclonal antibodies from hybridomas raised against bovine serum albumin conjugates of 0/sup 6/-n-butyldeoxyguanosine, 0/sup 2/-n-butylthymidine riboside and 0/sup 4/-n-butylthymidine riboside. The assays showed 50% inhibition of binding of specific (/sup 3/H)butyl-deoxynucleosides by 0.044, 0.069 and 0.45 pmole of cold 0/sup 6/-n-butyldeoxyguanosine, 0/sup 2/-n-butylthymidine and 0/sup 4/-n-butylthymidine respectively, corresponding to affinity constants of 2.7 x 10/sup 10/, 1.1 x 10/sup 10/ and 8.8 x 10/sup 8/ respectively. In competitive radioimmunoassays a similar degree of inhibition required approximately 10/sup 7/-fold higher concentrations of normal deoxynucleotides. From the relative inhibitions produced by a wide range of alkylated and normal nucleosides and bases it was concluded that each antibody primarily recognises butylation, as compared to other alkylations, at the sites specified by the immunogen. The radioimmunoassays should be suitable for the detection of these potentially promutagenic lesions in digests of DNA exposed to low (biological) levels of butylation.

• OSTI ID: 6717889
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:5; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKYLATING AGENTS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RIBOSIDES
SENSITIVITY
SPECIFICITY
TRACER TECHNIQUES
TRITIUM COMPOUNDS