Immunological detection of O/sup 6/-methylguanine in alkylated DNA

Journal Article · Tue May 16 04:00:00 EDT 1978 · Biochemistry; (United States)

OSTI ID:7057667

Briscoe, W T ^[1]; Spizizen, J; Tan, E M

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1. Scripps Clinic and Research Foundation, La Jolla, CA

Antibodies to O/sup 6/-methyldeoxyguanosine were produced in rabbits and utilized in a radioimmunoassay to detect this nucleoside at picomole levels. The specificity of the antibodies was demonstrated by the use of nucleoside analogues as inhibitors in the radioimmunoassay. The antibodies cross-reacted with O/sup 6/-methylguanosine, O/sup 6/-methylguanine, and O/sup 6/-ethylguanosine. There was 10/sup 4/ to 10/sup 6/ times less sensitivity to inhibition by deoxyadenosine, deoxyguanosine, and guanosine than by O/sup 6/-methyldeoxyguanosine. The radioimmunoassay also detected O/sup 6/-methylguanine in DNA alkylated by agents known to produce O/sup 6/-methylguanine, such as N'-methyl-N-nitrosourea. DNA alkylated with dimethyl sulfate, which does not produce O/sup 6/-methylguanine in DNA, cross-reacted with the antibodies to a very limited extent. Such an assay system for modified nucleic acid components would be very useful in following the production, persistence, and repair of these lesions in a variety of cells and tissues treated with a broad spectrum of carcinogens and suspected carcinogens.

• OSTI ID: 7057667
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 17:10; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560300 -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANTIBODIES
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BLOOD SERUM
CARCINOGENS
DNA
DRUGS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RIBOSIDES
SCREENING
TRACER TECHNIQUES
TRITIUM COMPOUNDS