Three-dimensional digital confocal Raman microscopy

Journal Article · Thu Dec 31 23:00:00 EST 1992 · Applied Spectroscopy; (United States)

DOI:https://doi.org/10.1366/0003702934048497· OSTI ID:6716402

Govil, A; Pallister, D M; Morris, M D ^[1]

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1. Univ. of Michigan, Ann Arbor (United States)

The authors describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging. 20 refs., 4 figs.

• DOE Contract Number: FG02-89ER13996
• OSTI ID: 6716402
• Journal Information: Applied Spectroscopy; (United States), Journal Name: Applied Spectroscopy; (United States) Vol. 47:1; ISSN 0003-7028; ISSN APSPA4
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
DATA ANALYSIS
DIGITAL FILTERS
IMAGE PROCESSING
LASER SPECTROSCOPY
MATHEMATICAL MODELS
MICROSCOPY
PROCESSING
RAMAN SPECTROSCOPY
SPECTROSCOPY
TRANSFORMATIONS