High definition Raman imaging. Final report, May 1, 1992--November 14, 1995
The authors have developed digital confocal Raman microscopy. In this 3-dimensional technique, a stack of Raman images is taken at intervals of 0.1--2 microns through the depth of the sample. The point spread function of the microscope is then deconvolved from the images, to yield a stack of sharply depth-resolved images. A constrained iterative deconvolution, which is computationally expensive, is used. The technique efficiently uses the available laser power and makes confocal Raman imaging possible. The procedure has been used on a number of polymeric samples, including polystyrene beads and polyester gratings, and shown to work well. The computation time has recently been reduced from about 45 minutes to about 2 minutes, using a digital signal processor (DSP) instead of the CPU of the general purpose workstation previously employed. In collaboration with a major glass maker, the authors have recently employed confocal Raman microprobe spectroscopy and imaging to identify and image potassium sulfate and molecular sulfur inclusions in glass pellets. The authors have also used the Raman spectrum of water as a non-invasive temperature probe in operating electrophoresis capillaries. The paper also describes a simple macro-scale imager that was constructed, explorations of holographic optical elements, and a micron-diameter silver probe for obtaining micron-resolved surface-enhanced Raman spectra.
- Research Organization:
- Michigan Univ., Ann Arbor, MI (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-89ER13996
- OSTI ID:
- 373831
- Report Number(s):
- DOE/ER/13996--T1; ON: DE96014911
- Country of Publication:
- United States
- Language:
- English
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