Beryllium-induced lung disease in the dog following two exposures to BeO

Conference · Tue Dec 01 04:00:00 EST 1992 · Environmental Research; (United States)

OSTI ID:6715620

Haley, P J; Finch, G L; Hoover, M D; Mewhinney, J A; Bice, D E; Muggenburg, B A ^[1]

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1. Lovelace Biomedical and Environmental Research Inst., Albuquerque, NM (United States)

The authors have shown previously that dogs exposed once to aerosols of beryllium oxide (BeO) calcined at 500 or 1,000[degrees]C developed granulomatous lung lesions as well as Be-specific immune responses in the blood and lung. In this report, they investigate the immunopathologic consequences of exposing dogs twice to aerosols of BeO. Dogs previously exposed to aerosols of 500 or 1,000[degrees]C calcined BeO to achieve an initial lung burden (ILB) of either 50 or 17 [mu]g/kg body wt were exposed a second time to BeO calcined at 500[degrees]C, 2.5 years after the first exposure, to achieve an ILB of about 50 [mu]g/kg body wt. Immune responses of peripheral blood and lung lymphocytes were measured at 0, 14, 30, 60, 90, 120, 150, 165, 180, and 210 days postexposure (dpe), and dogs were euthanized at 210 dpe. Be-specific immune responses occurred in blood at 30 dpe and again at 150 to 210 dpe. Only sporadic positive responses were seen among lung lymphocytes when cells were cultured in 10% fetal bovine serum. In contrast, samples collected at 165, 180, and 210 dpe and incubated with 10% dog serum showed a large number of positive responses in both blood and lung. Histologic lesions were characterized by perivascular and interstitial infiltrates of lymphocytes and macrophages with progression to patchy granulomatous pneumonia accompanied by focal septal fibrosis. The authors conclude that Be-induced granulomatous and fibrotic lung lesions are accompanied by Be-specific immune responses within the lung but these changes do not appear to be cumulative if enough time has elapsed between exposures. 20 refs., 8 figs., 3 tabs.

• DOE Contract Number: AC04-76EV01013
• OSTI ID: 6715620
• Report Number(s): CONF-9105413--
• Conference Information: Journal Name: Environmental Research; (United States) Journal Volume: 59:2
• Country of Publication: United States
• Language: English

Related Subjects

550900* -- Pathology
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BEAGLES
BERYLLIUM COMPOUNDS
BERYLLIUM OXIDES
BODY
BODY BURDEN
CHALCOGENIDES
CLEARANCE
DOGS
EXCRETION
IMMUNE REACTIONS
INHALATION
INTAKE
LUNG CLEARANCE
LUNGS
MAMMALS
MAXIMUM PERMISSIBLE BODY BURDEN
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
RESPIRATORY SYSTEM
SAFETY STANDARDS
STANDARDS
VERTEBRATES