Influence of DNA adenine methylase on the sensitivity of Escherichia coli to near-ultraviolet radiation and hydrogen peroxide

Journal Article · Thu May 31 04:00:00 EDT 1990 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(90)91433-S· OSTI ID:6715184

Yallaly, P; Eisenstark, A ^[1]

---

1. Univ. of Missouri, Columbia (USA)

Near-ultraviolet (NUV) radiation and hydrogen peroxide (H{sub 2}O{sub 2}) inactivation studies were performed on Escherichia coli K-12 DNA adenine methylation (dam) mutants and on cells that carry plasmids which overexpress Dam methylase. Lack of methylation resulted in increased sensitivity to NUV and H{sub 2}O{sub 2} (a photoproduct of NUV). In a dam mutant carrying a dam plasmid, the levels of Dam enzyme and resistance to NUV and H{sub 2}O{sub 2} were restored. However, using a multicopy dam+ plasmid strain, increasing the methylase above wildtype levels resulted in an increase in sensitivity of the cells rather than resistance.

• OSTI ID: 6715184
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 169:1; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
CELL CONSTITUENTS
CHEMICAL REACTIONS
DNA METHYLASES
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
FUNCTIONS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
LYASES
METHYLATION
MICROORGANISMS
NEAR ULTRAVIOLET RADIATION
NUCLEOPROTEINS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
PLASMIDS
PROTEINS
RADIATIONS
SENSITIVITY
ULTRAVIOLET RADIATION