A mutation in the dam gene of Vibrio cholerae: 2-aminopurine sensitivity with intact GATC methylase activity

Journal Article · Fri Dec 15 04:00:00 EST 1989 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/S0006-291X(89)80003-8· OSTI ID:5017887

Bandyopadhyay, R; Sengupta, A; Das, J ^[1]

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1. Indian Institute of Chemical Biology, Calcutta (India)

Vibrio cholerae mutants sensitive to 2-aminopurine (2AP) but with DNA adenine methylase activity similar to parental cells have been isolated. The mutant strains were sensitive to ultraviolet light (UV), methyl methanesulfonate (MMS) and 9-aminoacridine. The spontaneous mutation frequency of the mutants were not significantly affected. Attempts to isolate dam V. cholerae cells by screening 2AP sensitive cells have not been successful. All the mutant phenotypes could be suppressed by introducing the plasmid pRB103 carrying the dam gene of Escherichia coli into the mutant cells.

• OSTI ID: 5017887
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Journal Name: Biochemical and Biophysical Research Communications; (USA) Vol. 165:2; ISSN BBRCA; ISSN 0006-291X
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACRIDINES
AROMATICS
AZAARENES
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BACTERIA
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METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTATION FREQUENCY
MUTATIONS
NUCLEASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
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PHOSPHODIESTERASES
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RADIOINDUCTION
RADIOSENSITIVITY
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