Radioactive chromium-ethylenediaminetetraacetic acid for determination of glomerular filtration rate in rabbits

Journal Article · Tue Dec 01 04:00:00 EST 1981 · Lab. Anim. Sci.; (United States)

OSTI ID:6709225

Tvedegaard, E; Kamstrup, O

Simultaneous determination of the glomerular filtration rate was made by the insulin clearance technique and by analysis of the plasma disappearance curve of radioactive chromium-ethylenediaminetetraacetic acid (/sup 51/Cr-EDTA) following an intravenous injection in 28 anaesthetized rabbits. In some rabbits, the renal function was reduced by previous surgery. The insulin clearances ranged from 2.7 to 17 ml/minute. By a double-exponential analysis of the plasma disappearance curve, the total plasma clearance of /sup 51/Cr-EDTA was found to exceed the inulin clearance by 12%. By the simpler slope-intercept analysis requiring only two blood samples, this value was 32%. The renal clearance of /sup 51/Cr-EDTA was 97% of inulin clearance. In nephrectomized rabbits, plasma clearance of /sup 51/Cr-EDTA was 0.7 ml/minute. At all levels of renal function, measurement of the plasma clearance rate of /sup 51/Cr-EDTA by the single injection technique allowed a fairly reliable estimate of the glomerular filtration rate.

• Research Organization: Medical Department P 2131, Department of Experimental Pathology, Rigshospitalet, Copenhagen, Denmark
• OSTI ID: 6709225
• Journal Information: Lab. Anim. Sci.; (United States), Journal Name: Lab. Anim. Sci.; (United States) Vol. 31:6; ISSN LBASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
CLEARANCE
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
DYNAMIC FUNCTION STUDIES
EDTA
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
EXCRETION
FILTRATION
GLOMERULI
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
RABBITS
RADIOISOTOPES
RENAL CLEARANCE
SEPARATION PROCESSES
ULTRAFILTRATION
UROGENITAL SYSTEM DISEASES
USES
VERTEBRATES