Biopsy measurement of DNA damage and repair in vivo: single-strand breaks, alkali-labile bonds, and endonuclease-sensitive sites
Journal Article
·
· Radiat. Res.; (United States)
DNA damage and repair measurement by alkaline sucrose gradient sedimentation was adapted to in vivo biopsy samples by refining a sensitive DNA fluorescence assay and establishing quantitatible sedimentation conditions for high-molecular-weight DNA from isolated nuclei. Induction and repair of x-ray-induced single-strand breaks plus alkali-labile bonds in rat liver DNA were measured in whole liver and in successive biopsies taken from single animals; repair half-time was 20 to 30 min. Incubation of uv-irradiated rat liver nuclei with uv endonuclease before sedimentation enabled detection of pyrimidine dimers.
- Research Organization:
- Ohio State Univ., Columbus
- OSTI ID:
- 6707886
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 91:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA
Human repair endonuclease incises DNA at cytosine photoproducts
Pyrimidine dimer dependent cleavage of single-stranded DNA by T4 UV endonuclease
Journal Article
·
Fri May 01 00:00:00 EDT 1981
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:6123470
Human repair endonuclease incises DNA at cytosine photoproducts
Conference
·
Fri May 01 00:00:00 EDT 1987
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6019840
Pyrimidine dimer dependent cleavage of single-stranded DNA by T4 UV endonuclease
Journal Article
·
Tue Nov 25 23:00:00 EST 1986
· Biochem. Biophys. Res. Commun.; (United States)
·
OSTI ID:6928398
Related Subjects
560114 -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOPSY
BODY
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DNA
ELECTROMAGNETIC RADIATION
GLANDS
IN VIVO
IONIZING RADIATIONS
LIVER
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RATS
RECOVERY
REPAIR
RODENTS
SEDIMENTATION
STRAND BREAKS
VERTEBRATES
X RADIATION
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOPSY
BODY
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DNA
ELECTROMAGNETIC RADIATION
GLANDS
IN VIVO
IONIZING RADIATIONS
LIVER
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RATS
RECOVERY
REPAIR
RODENTS
SEDIMENTATION
STRAND BREAKS
VERTEBRATES
X RADIATION