Mechanisms for radiation damage in DNA: Progress report for the period June 1, 1988--May 31, 1989

Technical Report · Tue Nov 01 04:00:00 EST 1988

OSTI ID:6705904

Sevilla, M D

In this project we have proposed several mechanisms for radiation damage and recently radiation protection to DNA and its constituents, and have detailed a series of experiments utilizing electron spin resonance spectroscopy to test the proposed mechanisms. In the past we concentrated on investigating irradiated systems of DNA constituents. However, over the past several years we have included studies of spin labeled-DNA (and histone complexes), as well as radiation effects on sulfhydryls. In addition studies of radiation effects on lipids have been performed which shed light on the only other proposed site for cell kill, the membrane. In this years effort we have made several important scientific breakthroughs which have impact on the radiation protection provided by thiols as well as the oxygen enhancement effect. We have investigated the reactions of thiyl radicals with oxygen and found for the first time the thiol peroxyl radical and two other resulting sulfoxyl radicals which show differing chemistries. In addition the reactions of peroxyl radicals with thiols have been elucidated and we find this chemistry extends to DNA itself, i.e., DNA peroxyl radicals readily react with thiols.

• Research Organization: Oakland Univ., Rochester, MI (USA)
• DOE Contract Number: FG02-86ER60455
• OSTI ID: 6705904
• Report Number(s): DOE/ER/60455-3; ON: DE89003257
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AQUEOUS SOLUTIONS
BIOCHEMICAL REACTION KINETICS
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
DISPERSIONS
DNA
DOCUMENT TYPES
ELECTRON SPIN RESONANCE
KINETICS
LABELLING
MAGNETIC RESONANCE
MIXTURES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROBES
PROGRESS REPORT
RADIATION CHEMISTRY
RADIATION EFFECTS
RADICALS
RADIOLYSIS
REACTION KINETICS
RESONANCE
SOLUTIONS
STRUCTURE-ACTIVITY RELATIONSHIPS
THIYL RADICALS