Enhanced chromatid damage in blood lymphocytes after G2 phase x irradiation, a marker of the ataxia-telangiectasia gene

Journal Article · Wed Jun 20 00:00:00 EDT 1990 · JNCI, Journal of the National Cancer Institute; (USA)

DOI:https://doi.org/10.1093/jnci/82.12.1050· OSTI ID:6704845

Sanford, K K; Parshad, R; Price, F M; Jones, G M; Tarone, R E; Eierman, L; Hale, P; Waldmann, T A ^[1]

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1. National Cancer Institute, Bethesda, MD (USA)

An assay for ataxia-telangiectasia (A-T) heterozygotes, i.e., healthy carriers of the A-T gene(s), requiring only a small sample (3.5 mL) of peripheral blood, is described. Frequencies of chromatid aberrations in phytohemagglutinin-stimulated blood lymphocytes collected by demecolcine from 0.5 hour to 1.5 hours after x irradiation with 58 roentgens were twofold to threefold higher in A-T heterozygotes than in clinically normal controls and twofold to three-fold higher in A-T patients (homozygotes) than in A-T gene carriers. The persistence of chromatid breaks and gaps in lymphocytes following radiation-induced DNA damage during G2 suggests a deficiency or deficiencies in DNA repair that may be the defect at the molecular level that results in the enhanced radiosensitivity and cancer proneness characterizing A-T gene carriers and patients.

• OSTI ID: 6704845
• Journal Information: JNCI, Journal of the National Cancer Institute; (USA), Vol. 82:12; ISSN 0027-8874
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
LYMPHOCYTES
RADIOSENSITIVITY
TELANGIECTASIS
GENES
BIOLOGICAL MARKERS
CHROMATIDS
DNA
GENETIC RADIATION EFFECTS
MAN
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PATIENTS
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
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BLOOD
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BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
GENETIC EFFECTS
IONIZING RADIATIONS
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
SKIN DISEASES
SOMATIC CELLS
VASCULAR DISEASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture