Enhanced chromatid damage in blood lymphocytes after G2 phase x irradiation, a marker of the ataxia-telangiectasia gene

Journal Article · Wed Jun 20 04:00:00 EDT 1990 · JNCI, Journal of the National Cancer Institute; (USA)

DOI:https://doi.org/10.1093/jnci/82.12.1050· OSTI ID:6704845

Sanford, K K; Parshad, R; Price, F M; Jones, G M; Tarone, R E; Eierman, L; Hale, P; Waldmann, T A ^[1]

---

1. National Cancer Institute, Bethesda, MD (USA)

An assay for ataxia-telangiectasia (A-T) heterozygotes, i.e., healthy carriers of the A-T gene(s), requiring only a small sample (3.5 mL) of peripheral blood, is described. Frequencies of chromatid aberrations in phytohemagglutinin-stimulated blood lymphocytes collected by demecolcine from 0.5 hour to 1.5 hours after x irradiation with 58 roentgens were twofold to threefold higher in A-T heterozygotes than in clinically normal controls and twofold to three-fold higher in A-T patients (homozygotes) than in A-T gene carriers. The persistence of chromatid breaks and gaps in lymphocytes following radiation-induced DNA damage during G2 suggests a deficiency or deficiencies in DNA repair that may be the defect at the molecular level that results in the enhanced radiosensitivity and cancer proneness characterizing A-T gene carriers and patients.

• OSTI ID: 6704845
• Journal Information: JNCI, Journal of the National Cancer Institute; (USA), Journal Name: JNCI, Journal of the National Cancer Institute; (USA) Vol. 82:12; ISSN 0027-8874; ISSN JJIND
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MARKERS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMATIDS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ELECTROMAGNETIC RADIATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MUTATION FREQUENCY
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
SKIN DISEASES
SOMATIC CELLS
TELANGIECTASIS
VASCULAR DISEASES
VERTEBRATES
X RADIATION