Control of bovine hepatic fatty acid oxidation

Journal Article · Mon Sep 01 04:00:00 EDT 1986 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(86)80667-1· OSTI ID:6703443

Jesse, B W; Emery, R S; Thomas, J W

Fatty acid oxidation by bovine liver slices and mitochondria was examined to determine potential regulatory sites of fatty acid oxidation. Conversion of 1-(/sup 14/C)palmitate to /sup 14/CO/sub 2/ and total (/sup 14/C)acid-soluble metabolites was used to measure fatty acid oxidation. Oxidation of palmitate (1 mM) was linear in both liver slice weight and incubation time. Carnitine stimulated palmitate oxidation; 2 mM dl-carnitine produced maximal stimulation of palmitate oxidation to both CO/sup 2/ and acid-soluble metabolites. Propionate (10 mM) inhibited palmitate oxidation by bovine liver slices. Propionate (.5 to 10 mM) had no effect on palmitate oxidation by mitochondria, but malonyl Coenzyme A, the first committed intermediate of fatty acid synthesis, inhibited mitochondrial palmitate oxidation (inhibition constant = .3 ..mu..M). Liver mitochonndrial carnitine palmitoyltransferase exhibited Michaelis constants for palmitoyl Coenzyme A and l-carnitine of 11.5 ..mu..M and .59 mM, respectively. Long-chain fatty acid oxidation in bovine liver is regulated by mechanisms similar to those in rats but adapted to the unique digestive physiology of the bovine.

• Research Organization: Michigan State Univ., East Lansing
• OSTI ID: 6703443
• Journal Information: J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 69:9; ISSN JDSCA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARNITINE
CATTLE
CELL CONSTITUENTS
CHALCOGENIDES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
GLANDS
HEXADECANOIC ACID
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
METABOLITES
MITOCHONDRIA
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PROPIONIC ACID
RESPONSE MODIFYING FACTORS
RUMINANTS
TRACER TECHNIQUES
VERTEBRATES
VITAMIN B GROUP
VITAMINS