Influence of lipolysis on the mobilization of 2,4,5,2'4',5'-hexachlorobiphenyl from adipocytes in vitro
Epididymal adipocytes, isolated from rats pretreated with (/sup 14/C)-2,4,5,2',4',5'-hexachlorobiphenyl (6-CB), were utilized to examine the relationship between the mobilization of lipid and 6-CB and to determine whether 6-CB was differentially associated with subcellular organelles over time as has been demonstrated for newly synthesized lipid. Lipolysis, depleted approximately 1% of total cellular triacylglycerols (TG) regardless of time from treatment with 6-CB. The percentage of cellular 6-CB released from adipocytes to incubation buffer infranatants was not correlated with the magnitude of lipolysis produced over the 50-min incubation period; nor was the percentage of 6-CB released to the buffers correlated with the length of the incubation period, regardless of the presence of ISO. Although adipocytes responded similarly to lipolytic stimuli independent of time (days) since 6-CB treatment, significant decreases were found in the percentage of 6-CB released from adipocytes over time. The in vitro labeling of this newly synthesized TG in fat cells with (U-/sup 14/C)glucose or (1-/sup 14/C)palmitate demonstrated that TG was differentially distributed among adipocyte organelles. Newly synthesized TG was also the first to be mobilized under lipolytic stimulus. 6-CB was not released in a similar fashion, since radioactivity associated with the chemical levels of (/sup 14/C)-6-CB and glucose-derived /sup 14/C in buffers were not correlated over time.
- Research Organization:
- Medical College of Wisconsin, Milwaukee
- OSTI ID:
- 6702857
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Journal Name: J. Toxicol. Environ. Health; (United States) Vol. 20:1-2; ISSN JTEHD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
BIOLOGICAL LOCALIZATION
BODY
CARBON 14 COMPOUNDS
CHLORINATED AROMATIC HYDROCARBONS
CONNECTIVE TISSUE
DISTRIBUTION
HALOGENATED AROMATIC HYDROCARBONS
IN VITRO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RATS
RODENTS
SUBCELLULAR DISTRIBUTION
TISSUES
TRACER TECHNIQUES
VERTEBRATES