Effects of sublethal irradiation on changes in crypts of the mouse colon during treatment with 1,2-dimethylhydrazine
The effects of low-dose X-irradiation on the progression of early neoplastic changes induced in the colon by 1, 2-dimethylhydrazine (DMH) were assessed. Outbred female Swiss-Webster mice were treated with DMH only, DMH-irradiation, irradiation only, or 0.001 MEDTA. Animals were pulsed with (/sup 3/H)thymidine for 1 hour, and the distal colon was radioautographed. The number of labeled cells per crypt in animals tested with DMH-irradiation was greater (P less than 0.01) than the number in DMH-only animals, but a corresponding increase in the lenght of the crypts did not occur. After 16 weeks of treatment, the percentage of animals with focal atypiawas similar in the DMH-irradiation group (87%), and the DMH-only group (100%), but there were more focal atypias per animal in the DMH-only group. After 20 weeks, 14% of the DMH-irradiation group had visible tumors compared to 63% of the DMH-only group. The results suggest that DMH increased the number of cells at risk fro irradiation; thus the surge in proliferation subsequent to cell depetion was amplified. However, the development of precancerous lesions was not promoted.
- Research Organization:
- Department of Anatomy, School of Medicine, Oregon Health Sciences University, Portland
- OSTI ID:
- 6702562
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Vol. 69:3
- Country of Publication:
- United States
- Language:
- English
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HYDRAZINE
RADIOSENSITIVITY EFFECTS
LARGE INTESTINE
BIOLOGICAL RADIATION EFFECTS
AUTORADIOGRAPHY
EDTA
LOW DOSE IRRADIATION
MICE
NEOPLASMS
RADIOTHERAPY
THYMIDINE
TRITIUM COMPOUNDS
X RADIATION
AMINO ACIDS
ANIMALS
AZINES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
DIGESTIVE SYSTEM
DISEASES
ELECTROMAGNETIC RADIATION
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
INTESTINES
IONIZING RADIATIONS
IRRADIATION
LABELLED COMPOUNDS
MAMMALS
MEDICINE
NITROGEN COMPOUNDS
NUCLEAR MEDICINE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOLOGY
RIBOSIDES
RODENTS
THERAPY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals