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Transfer of experimental autoimmune thyroiditis with T cell clones

Journal Article · · J. Immunol.; (United States)
OSTI ID:6700448
;
We have investigated three T lymphocyte clones isolated from CBA/CaJ mice primed with mouse thyroid extract (MTE) in adjuvant. All three clones are L3T4+, Ig-, and Lyt2- and proliferate to MTE, mouse thyroglobulin (MTG) and rat thyroid extract. Clones A7 and B7 transfer thyroiditis to irradiated (475 rad) syngeneic mice, but not to normal recipients. The thyroid lesion induced by the B7 clone is characterized by the infiltration of both mononuclear and polymorphonuclear cells. The thyroiditis is transient in that lesions are apparent 7 and 14 days after transfer, but thyroids return to normal by day 21. Clone B7 showed helper activity for trinitrophenyl-keyhole limpet hemocyanin-primed B cells in vitro when stimulated with trinitrophenyl-MTG and also stimulated the production of anti-MTG antibody in recipient mice. Clone A7 induced thyroid lesions characterized by infiltration of the thyroid with mononuclear cells, with virtually no polymorphonuclear cell infiltration. This clone has shown no helper activity following stimulation with trinitrophenyl-MTG. The third clone (D2) proliferates to and shows helper activity to MTG, but fails to transfer thyroiditis to syngeneic, irradiated mice. On continuous culture, clone B7 lost its surface Thy. The loss of Thy appears unrelated to the ability to transfer thyroiditis since subclones of B7 with markedly different percentages of Thy+ cells transferred disease equally well.
Research Organization:
Scripps Clinic and Research Foundation, La Jolla, CA
OSTI ID:
6700448
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 4; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CHIMERAS
CLONE CELLS
CONNECTIVE TISSUE CELLS
DISEASES
ENDOCRINE DISEASES
GLOBULINS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MONOCYTES
MOSAICISM
NEUTROPHILS
ORGANIC COMPOUNDS
PROTEINS
RADIATION CHIMERAS
RATS
RODENTS
SOMATIC CELLS
THYROGLOBULIN
THYROIDITIS
TRANSPLANTS
VERTEBRATES