Augmentation of transfer of experimental autoimmune thyroiditis (EAT) in mice by irradiation of recipients

Journal Article · Thu Oct 15 04:00:00 EDT 1987 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(87)90322-4· OSTI ID:5665168

Williams, W V; Kyriakos, M; Sharp, G C; Braley-Mullen, H

Experimental autoimmune thyroiditis (EAT) can be adoptively transferred to normal syngeneic recipients using spleen cells from susceptible strains of mice primed in vivo with mouse thyroglobulin (MTg) and lipopolysaccharide (LPS) following in vitro activation of spleen cells by culture with MTg. Irradiation of recipient animals markedly augments the severity of thyroiditis induced in this system. Irradiation of recipients does not alter the time course of the development of thyroiditis, nor does it alter the requirement for both in vivo priming and in vitro activation of spleen cells for the development of EAT. Spleen cells from EAT-resistant strains of mice (e.g., Balb/c) do not induce EAT in irradiated recipients. Irradiated recipients develop significant levels of anti-MTg antibodies while unirradiated recipients have little detectable antibody response. The augmenting effect of irradiation can be substantially reversed by transferring naive spleen cells to recipients prior to the transfer of MTg/LPS-primed in vitro-activated spleen cells. In addition athymic CBA/Tufts nude mice develop more severe EAT than CBA/Tufts nude/+ littermates following transfer of activated CBA/J spleen cells. These data suggest that natural suppressor cells may regulate the development of EAT at the effector cell level.

• Research Organization: Univ. of Missouri, Columbia
• OSTI ID: 5665168
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 109:2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ENDOCRINE DISEASES
IMMUNE REACTIONS
IMMUNITY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
THYROIDITIS
TRANSPLANTS
VERTEBRATES