Development of a double-antibody radioimmunoassay for detecting ovarian tumor-associated antigen fraction OCA in plasma
Journal Article
·
· Am. J. Obstet. Gynecol.; (United States)
OSTI ID:6700123
Ovarian tumor-associated antigen isolated from human tumor tissue was shown to have a different mobility from that of carcinoembryonic antigen (CEA) in both acrylamide gel electrophoresis and immunoelectrophoresis in agarose. The ovarian tumor antigen is composed of six species with different electrophoretic mobility in acrylamide gel electrophoresis. Three of these species were detected in Sephadex G-100 ovarian fraction OCA (from the void volume peak) and the other three species of lower apparent molecular weight were detected in fraction OCD (from the second peak). Fractions OCA and OCD did not share common antigenic determinants as determined by immunodiffusion. CEA was shown to share antigenic determinants with both OCA and OCD. A double-antibody radioimmunoassay capable of detecting nanogram quantities of plasma OCA was developed. In a preliminary study of ovarian cancer patients, OCA appeared to be a more sensitive marker for ovarian cancer than CEA. There was virtually no correlation (r/sup 2/ = 0.1) between OCA and CEA levels in these patients, as determined by radioimmunoassay.
- Research Organization:
- Univ. of Toronto
- OSTI ID:
- 6700123
- Journal Information:
- Am. J. Obstet. Gynecol.; (United States), Journal Name: Am. J. Obstet. Gynecol.; (United States) Vol. 131:7; ISSN AJOGA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550901 -- Pathology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GONADS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OVARIES
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
550901 -- Pathology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
GONADS
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OVARIES
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES