Effect of albumin-bound DHA on phosphoinositide phosphorylation in collagen stimulated human platelets

Journal Article · Tue May 15 04:00:00 EDT 1990 · Thrombosis Research; (USA)

DOI:https://doi.org/10.1016/0049-3848(90)90214-W· OSTI ID:6698745

Gaudette, D C; Holub, B J ^[1]

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1. Univ. of Guelph, Ontario (Canada)

The effect of exogenous albumin-bound docosahexaenoic acid (22:6n-3) (DHA), arachidonic acid (20:4n-6) (AA), and eicosapendaenoic acid (20:5n-3) (EPA) on phosphoinositide metabolism following collagen stimulation was studied using (3H)inositol prelabelled platelets. Collagen stimulation (3 min, 1.8 micrograms/ml) increased the labelling of both phosphatidylinositol 4-monophosphate (PIP), and phosphatidylinositol 4,5-biphosphate (PIP2). Of the fatty acids tested, only pre-incubation (2 min) with DHA (20 microM) significantly attenuated the collagen-induced increased PIP and PIP2 labelling; EPA was without effect, while AA enhanced PIP labelling. Forty microM DHA was less effective at attenuating the increased PIP and PIP2 labelling even though this concentration of DHA resulted in greater inhibition of platelet aggregation. Neither concentration of DHA attenuated the increased polyphosphoinositide labelling resulting from stimulation by the endoperoxide analogue U46619, or the phorbol ester, PMA. These data suggest that the effect of DHA on attenuating the increased PIP and PIP2 labelling following collagen stimulation likely occurs before thromboxane receptor occupancy, may not occur at the level of protein kinase C activation, and could be mediated in part via a lessened synthesis of thromboxane A2.

• OSTI ID: 6698745
• Journal Information: Thrombosis Research; (USA), Journal Name: Thrombosis Research; (USA) Vol. 58:4; ISSN THBRA; ISSN 0049-3848
• Country of Publication: United States
• Language: English

Related Subjects

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560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
CHEMICAL REACTIONS
COLLAGEN
ESTERS
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LIPIDS
MAMMALS
MAN
MATERIALS
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHORBOL ESTERS
PHOSPHOLIPIDS
PHOSPHORYLATION
PRIMATES
PROSTAGLANDINS
PROTEINS
REACTION KINETICS
SCLEROPROTEINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES