The effects of the oral administration of fish oil concentrate on the release and the metabolism of (/sup 14/C)arachidonic acid and (/sup 14/C)eicosapentaenoic acid by human platelets

Journal Article · Mon Nov 01 04:00:00 EST 1982 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(82)90112-8· OSTI ID:6257823

Hirai, A; Terano, T; Hamazaki, T; Sajiki, J; Kondo, S; Ozawa, A; Fujita, T; Miyamoto, T; Tamura, Y; Kumagai, A

It has been suggested by several investigators that eicosapentaenoic acid (C20:5 omega 3, EPA) might have anti-thrombotic effects. In this experiment, the effect of the oral administration of EPA rich fish oil concentrate on platelet aggregation and the release and the metabolism of (/sup 1 -14/C)arachidonic acid and ((U)-/sup 14/C)eicosapentaenoic acid by human platelets was studied. Eight healthy male subjects ingested 18 capsules of fish oil concentrate (EPA 1.4 g) per day for 4 weeks. Plasma and platelet concentrations of EPA markedly increased, while those of arachidonic acid (C20:4 omega 6, AA) and docosahexaenoic acid (C22:6 omega 3, DHA) did not change. Platelet aggregation induced by collagen and ADP was reduced. Collagen induced (/sup 14/C)thromboxane B2 (TXB2) formation from (/sup 14/C)AA prelabeled platelets decreased. There was no detectable formation of (/sup 14/C)TXB3 from (/sup 14/C)EPA prelabeled platelets, and the conversion of exogenous (/sup 14/C)EPA to (/sup 14/C)TXB3 was lower than that of (/sup 14/C)AA to (/sup 14/C)TXB2. The release of (/sup 14/C)AA from (/sup 14/C)AA prelabeled platelets by collagen was significantly decreased. These observations raise the possibility that the release of arachidonic acid from platelet lipids might be affected by the alteration of EPA content in platelets.

• Research Organization: Department of Internal Medicine, School of Medicine, Chiba University, Japan
• OSTI ID: 6257823
• Journal Information: Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 28:3; ISSN THBRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AGGLOMERATION
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
EICOSANOIC ACID
FISH OIL
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MATERIALS
METABOLISM
MONOCARBOXYLIC ACIDS
NORTH AMERICA
OILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OTHER ORGANIC COMPOUNDS
QUANTITY RATIO
REACTION KINETICS
TRACER TECHNIQUES
USA