Plasma half-life and organ uptake ratio of radiolabeled glandular kallikrein in control and nephrectomized rats

Journal Article · Wed Jan 01 04:00:00 EST 1986 · Adv. Exp. Med. Biol.; (United States)

DOI:https://doi.org/10.1007/978-1-4684-5143-6_42· OSTI ID:6698355

Nishimura, K; Iwata, T; Kokubu, T

The purified rat urinary kallikrein was radiolabeled by lactoperoxidase method and by chloramine T method. Plasma half-life of radiolabeled kallikrein was 5.06 +/- 0.59 (n = 5) min in control rats and 5.24 +/- 0.42 (n = 5) min in nephrectomized rats. There was no difference between two groups. From autoradiogram, main metabolic organs of radiolabeled kallikrein were liver, kidney and spleen. Total uptake of radiolabeled kallikrein in ech organ was the highest in liver (73.2%). The uptake per g tissue of radiolabeled kallikrein in each organ was high in liver (33.0%), kidney (31.4%) and spleen (21.1%). These results suggest that the active kallikrein is metabolized mainly in the liver, and kidney is not so an important organ to metabolize or to eliminate the active kallikrein in plasma. In order to clarify the mode of existence of active kallikrein in plasma, the following experiment was done by using disc gel electrophoresis. Radioactive profile of radiolabeled kallikrein showed one peak (Rf = 1.0), but radiolabeled kallikrein mixed with rat plasma showed two peaks, that is small peak (Rf = 1.0), and main peak (RF = 0.5). The most of radiolabeled kallikrein was bound to plasma protein and only five per cent was in free form. Furthermore, the binding of radiolabeled kallikrein to plasma protein was interfered by the addition of active kallikrein. These results suggest the possibility of existence of kallikrein binding protein in plasma.

• Research Organization: Ehime Univ. School of Medicine, Japan
• OSTI ID: 6698355
• Journal Information: Adv. Exp. Med. Biol.; (United States), Journal Name: Adv. Exp. Med. Biol.; (United States); ISSN AEMBA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD COAGULATION FACTORS
BLOOD PLASMA
BODY
BODY FLUIDS
COAGULANTS
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
ENZYMES
GLANDS
HEMATOLOGIC AGENTS
HYDROLASES
ISOTOPE APPLICATIONS
KIDNEYS
KININOGENIN
LABELLED COMPOUNDS
LIVER
MAMMALS
MATERIALS
MEDICINE
NEPHRECTOMY
ORGANS
PEPTIDE HYDROLASES
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SERINE PROTEINASES
SPLEEN
SURGERY
TISSUE DISTRIBUTION
TRACER TECHNIQUES
UPTAKE
VERTEBRATES