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Immunoreactive glandular kallikrein in rat plasma: a radioimmunoassay for its determination

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:5451100
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A radioimmunoassay (RIA) has been developed to measure immunoreactive glandular kallikrein in rat plasma. To prevent the binding of radioactive kallikrein to plasma inhibitors, /sup 125/I-kallikrein was inactivated with phenylmethylsulfonyl fluoride (PMSF), a procedure that maintained /sup 125/I-kallikrein immunoreactivity. Different volumes of plasma displaced /sup 125/I-PMSF-kallikrein in a parallel fashion to the kallikrein standard curve. The sensitivity of the RIA was 200 pg, and the recovery of nonradioactive active kallikrein added to plasma was 58.7%. The concentration of immunoreactive glandular kallikrein in normal rat plasma averaged 47.1 +/- 1.7 (SE) ng/ml. Bilateral nephrectomy caused a threefold increase in circulating glandular kallikrein (50 +/- 2.7 to 167 +/- 7 ng/ml; P < 0.001). Removal of the submandibular and sublingual glands signficantly decreased its concentration from 52 +/- 2.3 to 34 +/- 1.6 ng/ml (P < 0.001). Immunoreactive glandular kallikrein was higher in the submandibular gland vein than in arterial blood (venous; 94 +/- 10.5; arterial: 64 +/- 6.3 ng/ml; P < 0.05) and was lower in the renal venous blood (venous: 44 +/- 2.2; arterial: 53 +/- 2.6 ng/ml; P < 0.05). In conclusion, this study shows that the use of /sup 125/I-PMSF-kallikrein as tracer prevents the interference in the RIA caused by plasma protease inhibitors. It also indicates that the submandibular gland is an important source of the immunoreactive glandular kallikrein in rat plasma and that the kidney probably participates in its metabolism. Glandular kallikrein released by the submandibular gland into the circulation may participate in regulating local blood flow before it is inactivated by plasma inhibitors.

Research Organization:
Henry Ford Hospital, Detroit, MI
OSTI ID:
5451100
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 242:4; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD COAGULATION FACTORS
BLOOD PLASMA
BODY
BODY FLUIDS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FLUORIDES
FLUORINE COMPOUNDS
GLANDS
HALIDES
HALOGEN COMPOUNDS
HEMATOLOGIC AGENTS
HYDROLASES
INACTIVATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KININOGENIN
MAMMALS
MATERIALS
MEDICINE
NEPHRECTOMY
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
SALIVARY GLANDS
SERINE PROTEINASES
SULFUR COMPOUNDS
SULFUR FLUORIDES
SURGERY
TRACER TECHNIQUES
VERTEBRATES